Sealed capillary tubes hold the samples, which can be up to 35 µl. Sealed tubes also provide safety and easy storage. Eight samples, plus a reference, are tested in approximately one minute.
Ultra-Sensitive Spectrofluorometer: SignalyteTM-II
SignalyteTM-II is an ultra-sensitive spectrofluorometer that is up to 1000 times more sensitive than a fluorescent plate reader.
SignalyteTM-II is an ultra-sensitive endpoint fluorescence reader that can be used in medical diagnostics as a biosensor for detecting proteins, bacteria, viruses, toxins, disease, drugs and other biomarkers in saliva, urine, breath, sweat, blood, food, water and environmental samples. SignalyteTM-II can also be used to identify DNA and RNA.
- Enzymatic assays
- Detection of viruses, bacteria, spores, proteins, toxins, disease markers
- Detection of DNA and RNA without PCR
- FRET assay
- ATP assay
- dsDNA and end point PCR reader
- Reporter gene assays
- Cell proliferation
- Toxicity assay
Many immunoassays use magnetic beads to concentrate and remove target analyte from the matrix; Creatv employs this method in its test kit for E. coli O157:H7. The detection limit is 10 cfu/ml using SignalyteTM-II to read the fluorescence signal. This magnetic bead protocol can also be applied to many other analytes.
The SignalyteTM-II spectrofluorometer is up to 1000 times more sensitive than fluorescent plate readers, depending on the dye and assay.