CellSieveTM CTC Enumeration Kit
Contains filters, buffers, and stains for 10 assays
For Research Use Only. This product is not intended for in vivo or diagnostic
use. Performance, safety, and effectiveness have not been established and
this kit is not approved by the FDA.
Standard Markers
- DAPI (positive) for the nuclear
- CK 8, 18, 19 (positive) to identify as epithelial cell
- CD 45 (negative) to show it is not a blood cell
Fourth Fluorescent Channel
- Monochrome
- Standard kit: EpCAM
- Can be customized
Camera requirement
- Sensitive to NIR
- 1.4M pixels minimum
Microscope Filter Set Specifications
Fluorescence Channel |
Chroma Part # |
Excitation Filter |
Emission Filter |
Dichroic Filter |
DAPI
|
49000 |
350/50
|
460/50 |
400
|
FITC
|
49020
|
480/20
|
510/20 |
495 |
TRITC/PE
|
CellSieve-PE
|
546/10
|
585/40
|
565
|
Texas Red
|
49911
|
594/10
|
645/75
|
594
|
Cyanine5
|
CellSieve-Cy5
|
640/30
|
700/75
|
660
|
Materials
- 10 CellSieveTM Microfilters
- CellSieveTM Prefixation Buffer
- CellSieveTM Postfixation Buffer
- CellSieveTM Permeabilization Buffer
- Ten White Gaskets and Ten Black Gaskets
- CellSieveTM Enumeration Staining Solution
- CellSieveTMMounting Solution with DAPI dilactate