Escherichia coli O157 Test Kit

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Protocol

E. coli Scientific Publications and Presentations

Rapid and Sensitive Isolation and Identification of

E. coli O157:H7

By combining immunocapture and fluorescence immunoassay, this E.coli O157:H7 test kit is able to provide both high sensitivity and specificity. Limit of detection using SignalyteTM-II is 10 cfu/ml.

Applications

  • Water
  • Food
  • Environment

Assay Steps

  • Isolation and concentration of E. coli O157:H7 from sample using immunoaffinity magnetic beads
  • Detection using fluorometer, such as
    • Creatv’s SignalyteTM-II spectrofluorometer
    • 96 well fluorescent plate readers


(A)


(B)

Fluorescence immunoassay for the detection of a 10-fold serial E. coli O157:H7 dilution. (A) SignalyteTM-II; (B) 96 well fluorescence plate reader. The y-axis indicates the log10 fluorescence intensity (FI), while x-axis indicates log10 concentration of E. coli O157:H7 (cfu/ml). The threshold value of fluorescence intensity for the limit of detection is the average Fl plus 3 standard deviations of the negative control FI.