Detection Principle

Integrating Waveguide Technology

The principle of detection consists of illuminating the cuvette at a 90º angle relative to the length of the waveguide, and subsequent collection of the emitted fluorescence from the sealed end. The emitted light is efficiently gathered by the cuvette and guided by the cuvette/sample as a waveguide.

The emission signal exits from the end of the waveguide, through lenses and filters to the optical detector. Emitted light from the entire waveguide is integrated, thus maximizing the detected signal. Background noise from the excitation light is minimized by the 90º excitation angle. Signal-to-noise ratio is improved compared to other test geometries, enabling more sensitive assays.